Elimination of 3:4-benzpyrene from the rat

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Abstract
On alternate days 33 rats were injected intravenously with 1-2 cc. of a 0.03% benzpyrene colloid. The extraction of a derivative of benzpyrene from the feces and its purification by chromatographic adsorption on Al2O3 are described. The product is photolabile and soluble in NaOH with green-yellow fluorescence; an alcoholic solution shows 2 diffuse fluorescence bands in the blue-violet region of the spectrum. Benzpyrene is eliminated in the urine as a fluorescent derivative and as a glucuronate. The fluorescent substance in the urine was apparently present in smaller quantity than that in the feces.