The Nuclear Autoimmune Antigen Ku IS Also Present on the Cell Surface
- 1 January 1992
- journal article
- research article
- Published by Taylor & Francis in Autoimmunity
- Vol. 13 (4) , 265-267
- https://doi.org/10.3109/08916939209112334
Abstract
Polyclonal antibodies were raised against the individual 85 and 70 kDa subunits of the Ku complex purified from nuclear extract prepared from the T cell line MLA 144. They specifically recognise the appropriate subunits of the Ku complex from whole cell extract of HeLa cells using Western blot analysis. They are also able to identify the Ku proteins present in the cell membrane using FACS analysis.Keywords
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