Evaluation of thrombus detection in a rabbit model using a technetium‐99M‐labelled anti‐fibrin monoclonal antibody

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Abstract
Summary: Technetium‐99 m (99mTc)‐labelled conjugates of an anti‐fibrin monoclonal antibody, DD‐3B6/22, have been assessed for their detection of vascular thrombi in a rabbit model. DD‐3B6/22 binds to a D‐dimer epitope present on cross‐linked fibrin but absent from the fibrin monomer or fibrinogen. Injection of a 99mTc‐label led Fab′ fragment of DD‐3H6/22 allowed delineation of model thrombi as early as 30 min postinjection (p.i.) with optimal localization at 4–5 h. Thrombus label uptake at 4 h p.i. was 0.304 ± 0.106% injected dose/g (% ID/g) compared with 0.022 ± 0.001% ID/g after the injection of a control Fab′ fragment. These results suggest that the 99mTc‐labelled Fab′ fragment of DD‐3B6/22 has excellent potential for scintigraphic detection of vascular thrombi in humans.