Regulatory pattern of the Escherichia coli lysA gene: expression of chromosomal lysA-lacZ fusions
- 1 December 1983
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 156 (3) , 1198-1203
- https://doi.org/10.1128/jb.156.3.1198-1203.1983
Abstract
The regulation of lysA which encodes the last enzyme for lysine biosynthesis in Escherichia coli, diaminopimelic acid-decarboxylase, was studied by using lysA-lacZ fusions. Our results indicate an absolute requirement for the LysR product for its activation, LysR protein present in a limiting amount which can be titrated by a multicopy plasmid carrying its target site and a negative regulatory role for the LysA protein itself which decreases lysA-lacZ expression 30-fold.This publication has 18 references indexed in Scilit:
- Regulation of diaminopimelate decarboxylase synthesis in Escherichia coliJournal of Molecular Biology, 1983
- Regulation of diaminopimelate decarboxylase synthesis in Escherichia coliJournal of Molecular Biology, 1983
- Control of ColE1 DNA replication: the rop gene product negatively affects transcription from the replication primer promoter.Proceedings of the National Academy of Sciences, 1982
- Construction of hybrid plasmids containing the lysA gene of Escherichia coli: Studies of expression in Escherichia coli and Saccharomyces cerevisiaeMolecular Genetics and Genomics, 1981
- The relA locus and the regulation of lysine biosynthesis in Escherichia coliMolecular Genetics and Genomics, 1980
- Isolation and identification of mutants constitutive for aspartokinase III synthesis in Escherichia coli K 12Biochimie, 1980
- Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences.Proceedings of the National Academy of Sciences, 1979
- Multiple regulation of DAP-decarboxylase synthesis inEscherichia coliK-12FEMS Microbiology Letters, 1979
- Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and MuJournal of Molecular Biology, 1976
- A simple method for the preparation of large quantities of pure plasmid DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975