Inhibition of Leukocyte Migration: An Evaluation of this in Vitro Assay of Delayed Hypersensitivity in Man to a Soluble Antigen
Open Access
- 1 December 1970
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 105 (6) , 1447-1452
- https://doi.org/10.4049/jimmunol.105.6.1447
Abstract
An in vitro assay of delayed hypersensitivity in man, modified from the method of Søborg and Bendixen, was evaluated. This assay is based on the inhibition of migration of human peripheral leukocytes in the presence of specific soluble antigen. Twelve of 13 individuals who were skin test positive to tuberculin showed inhibition of leukocyte migration when incubated with 100 µg/ml of soluble purified protein derivative. Only 1 of 12 skin test negative individuals showed a similar inhibition of leukocyte migration. Analysis of the migrating populations showed that the migration of mononuclear cells and not of neutrophils was inhibited.Keywords
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