Generation of Epstein‐Barr Virus (EBV)–Immortalized B Cell Lines
Open Access
- 1 February 2007
- journal article
- unit
- Published by Wiley in Current Protocols in Immunology
- Vol. 76 (1) , 7.22.1-7.22.4
- https://doi.org/10.1002/0471142735.im0722s76
Abstract
Immortalization of B lymphocytes by EBV is an effective procedure for inducing long‐term growth of certain human B lymphocytes. The protocol described in this unit to accomplish this can be divided into three stages: preparation of virus, preparation of target cells to be immortalized, and EBV infection and growth of infected cells.Keywords
This publication has 13 references indexed in Scilit:
- High-potentiality preliminary selection criteria and transformation time-dependent factors analysis for establishing Epstein?Barr virus transformed human lymphoblastoid cell linesCell Proliferation, 2006
- Transmissible Retrovirus in Epstein-Burr Virus-Producer B95-8 CellsVirology, 1995
- A method for transforming lymphocytes from very small blood volumes suitable for paediatric samplesHuman Genetics, 1991
- A routine method for the establishment of permanent growing lymphoblastoid cell linesHuman Genetics, 1986
- Epstein-Barr virus susceptibility of normal human B lymphocyte populations.The Journal of Experimental Medicine, 1984
- Selective inhibition of immunoregulatory cell functions by cyclosporin A.The Journal of Immunology, 1982
- Phenotypic and Cytogenetic Characteristics of Human B-Lymphoid Cell Lines and Their Relevance for the Etiology of Burkitt'S LymphomaPublished by Elsevier ,1982
- Long-term T-cell-mediated immunity to Epstein-Barr virus in man. II. Components necessary for regression in virus-infected leukocyte culturesInternational Journal of Cancer, 1979
- Release of Infectious Epstein-Barr Virus by Transformed Marmoset LeukocytesProceedings of the National Academy of Sciences, 1973