Estimation of Urinary Reducing Corticosteroids with Blue Tetrazolium

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Abstract
DETERMINATION of the concentration of the corticosteroids in urine has become an increasingly important problem. A variety of methods have been proposed for their estimation, based on the reducing properties of the alpha-ketol side chain at C17, on the formaldehydogenic potential of this side chain or on empiric, poorly understood color reactions of this molecular species.The most reliable method is that of Talbot and his associates,1 which, although complicated and therefore impractical for general use, has the distinct advantage of nearly proving the main structural features of the corticosteroids in each determination. The steps of the procedure . . .