Interleukin 2 receptor expression in unstimulated murine splenic T cells. Localization to L3T4+ cells and regulation by non-H-2-linked genes.
Open Access
- 1 June 1986
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 163 (6) , 1376-1390
- https://doi.org/10.1084/jem.163.6.1376
Abstract
The present study reports the surprising observation that IL-2-R+ cells can be detected in fresh, unstimulated, murine spleen T cells from unimmunized mice by flow cytometry using the monoclonal anti-receptor antibody 7D4. Also, unexpectedly, these cells were found exclusively in the L3T4+Lyt-2- population by two-color fluorescence, in contrast to receptor+ cells after stimulation, in which both L3T4+Lyt-2- and Lyt-2+L3T4- cells were found. The fraction of splenic T cells bearing IL-2-R reproducibly varies twofold under non-H-2-linked genetic control, with high expression in DBA/2 and BALB/c (approximately 6-7%) and low expression in B10.D2 and C57BL/6 (3%). This correlates quantitatively with a greater responsiveness of the DBA/2 and BALB/c splenic T cells to high doses of IL-2, compared with B10.D2 T cells; twice as many B10.D2 T cells as DBA/2 T cells were required to get the same response. Studies with 23 B X D RI strains revealed that the level of IL-2-R+ cells in unstimulated spleen cells was regulated by multiple genes, very likely including at least one gene on chromosome 7, near the HBB locus. The mapping makes novel use of nonparametric (Smirnov) statistics, which we suggest may be of general usefulness in similar analyses of RI strains.This publication has 26 references indexed in Scilit:
- Interleukin 2Annual Review of Immunology, 1984
- Functional analysis of human T cell subsets defined by monoclonal antibodies. I. Collaborative T-T interactions in the immunoregulation of B cell differentiation.The Journal of Immunology, 1980
- In vitro growth of cytotoxic human lymphocytes. II. Use of T cell growth factor (TCGF) to clone human T cells.The Journal of Immunology, 1980
- Ligand-Activated T Cell Growth Factor-Induced Proliferation: Absorption of T Cell Growth Factor by Activated T CellsThe Journal of Immunology, 1979
- Separation of functional subsets of human T cells by a monoclonal antibody.Proceedings of the National Academy of Sciences, 1979
- Studies on T lymphocyte activation II. The target cells for concanavalin A‐induced growth factorsEuropean Journal of Immunology, 1979
- Rapid data collection, analysis, and graphics for flow microfluorometry instrumentationReview of Scientific Instruments, 1978
- Functional and Morphologic Characterization of Human T Cells Continuously Grown in VitroThe Journal of Immunology, 1977
- The Mls system.1976
- A rapid method for the isolation of functional thymus‐derived murine lymphocytesEuropean Journal of Immunology, 1973