Gonadotropin-Induced Inhibition of Oxygen Consumption in Rat Oocyte-Cumulus Complexes: Relief by Adenosine1

Publisher Website
Google Scholar
Abstract
In the cumulus-oocyte complex (COC), as well as in the whole ovarian follicle, gonadotropins stimulate glycolysis, measured as lactate accumulation. In contrast to this, COC oxyten consumption is decreased by gonadotropins. One possible explanation for this inhibition is a competition for a limited supply of cofactors, common for both the glycolytic pathway and the respiratory chain. In the present study, addition of adenosine to COCs cultured with gonadotropins restored the oxygen consumption to basal levels. This effect was specific for metabolizable adenosine. Other nucleosides or non-metabolizable 2-chloro-adenosine had no effect. Adenosine also increased ATP and decreased lactate accumulation by the COC. The nucleoside transport inhibitor dipyridamol abolished the effects of adenosine on oxygen consumption and ATP accmulation. Taken together these results support the hypothesis that a relative lack of adenosine-derived cofactors is probably involved in the decrease of oxygen consumption in vitro by gonadotropin-stimulated cumulus cells.