The composition of isolated cerebral tissue: purines

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Abstract
Levels of adenine, guanine and hypoxanthine nucleotides in the whole brains of guinea pigs immersed in liquid nitrogen were 2.95, 0.65 and 0.1 [mu]moles/g of tissue respectively. Adeno-sine was at 0.3 and hypoxanthine at 0.1 [mu]mole/g of tissue. Cortex slices fixed immediately gave 1.64, 0.38 and 0.1 [mu]moles/g of tissue for adenine, guanine and hypoxanthine nucleotides respectively. Adeno-sine was at 0.75, inosine at 0.2 and hypoxanthine at 0.2 [mu]mole/g of tissue. Slices incubated in oxygenated glucose-containing medium maintained adenine and guanine nucleotides at 0.89 and 0.17 [mu]mole/g of wet tissue respectively. Additions of adenine, guanine, hypoxanthine and creatine at 1 uM to the medium had little effect on nucleotide content of incubated slices. Slices respiring in medium containing 1 [mu][image]-adenosine raised adenine nucleotides to 1.27 and added guanosine increased guanine nucleotide to 0.21 [mu]mole/g of wet tissue. In a mixture of adenosine, guanosine and creatine at 1 u[image] in medium 1.56 and 0.27 [mu]moles of adenine and guanine nucleotides/g of wet tissue were resynthesized in slices incubated for 3 hours.