Abstract
The Japanese firefly squid, Watasenia scintillans , emits intense flashes of light from three tiny luminous organs that are located at the tip of each of a pair of ventral arms. Light is also produced from hundreds of other minute organs that are scattered over the body. The luminescence is due to an ATP-dependent reaction, with an optimal pH of 8.80. The decay of light intensity follows first-order kinetics and the decay constant is independent of initial ATP concentration. The light emission also requires MgCl 2 , a soluble component, and an insoluble component that is membrane bound. Squids represent a major group of organisms unrelated to fireflies in which ATP is required for bioluminescence.