Dual Role of Phosphatidylinositol-3,4,5-trisphosphate in the Activation of Protein Kinase B
- 25 July 1997
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 277 (5325) , 567-570
- https://doi.org/10.1126/science.277.5325.567
Abstract
Protein kinase B (PKB) is a proto-oncogene that is activated in signaling pathways initiated by phosphoinositide 3-kinase. Chromatographic separation of brain cytosol revealed a kinase activity that phosphorylated and activated PKB only in the presence of phosphatidylinositol-3,4,5-trisphosphate [PtdIns(3,4,5)P3]. Phosphorylation occurred exclusively on threonine-308, a residue implicated in activation of PKB in vivo. PtdIns(3,4,5)P3 was determined to have a dual role: Its binding to the pleckstrin homology domain of PKB was required to allow phosphorylation by the upstream kinase and it directly activated the upstream kinase.Keywords
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