ALTERNATIVE COMPLEMENT PATHWAY IN BOVINE SERUM - LYSIS OF HUMAN ERYTHROCYTES

  • 1 January 1977
    • journal article
    • research article
    • Vol. 38  (3) , 355-359
Abstract
The hemolysis of unsensitized human erythrocytes by fresh bovine serums was investigated. Lysis occurred in ethylene glycol bis-amino tetraacetate buffers and with serums depleted of C1q [q fragment of the 1st complement component]. Serums extensively absorbed with packed human erythrocytes at 0.degree. C effectively lysed human erythrocytes, but optimal lytic capacity required target cells sensitized with a heat-stable serum factor. Lysis did not occur with serums absorbed with zymosan at 17.degree. C or heat inactivated at 50.degree. C. Human erythrocytes can apparently activate the alternative pathway of C in bovine serums. Lysis can proceed in the apparent absence of antibodies, although their presence may enhance the reaction.

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