Neutralizing monoclonal antibody specific for α‐bungarotoxin: Preparation and characterization of the antibody, and localization of antigenic region of α‐bungarotoxin
- 28 August 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 254 (1-2) , 106-110
- https://doi.org/10.1016/0014-5793(89)81018-x
Abstract
We prepared an α-bungarotoxin-specific monoclonal antibody that neutralizes the biological activity of the toxin in vivo. The antigenic determinant combining specifically with this antibody was determined on the basis of cross-reaction experiments using three other long neurotoxins and peptide fragments of α-bungarotoxin. The antigenic determinant was located on the peptide fragment containing S34-S35-R36-G37-K38, which forms a part of the expected site that binds to the acetylcholine receptor proteinKeywords
This publication has 12 references indexed in Scilit:
- Isolation of antigenically reactive peptide fragments and localization of antigenic regions of α-bungarotoxinBiochemical and Biophysical Research Communications, 1988
- Structural studies of .alpha.-bungarotoxin. 1. Sequence-specific proton NMR resonance assignmentsBiochemistry, 1988
- Primary structure of α‐bungarotoxin Six amino acid residues differ from the previously reported sequenceFEBS Letters, 1987
- Current view on the structure-function relationship of postsynaptic neurotoxins from snake venomsPharmacology & Therapeutics, 1987
- Molecular conformation of α-bungarotoxin as studied by nuclear magnetic resonance and circular dichroismJournal of Molecular Biology, 1985
- Conformational Properties of the Neurotoxins and Cytotoxins Isolated from Elapid Snake VenomsCritical Reviews in Biochemistry, 1983
- Neutralizing monoclonal antibody specific for Naja nigricollis toxin .alpha.: preparation, characterization and localization of the antigenic binding siteBiochemistry, 1982
- [1] Preparation of monoclonal antibodies: Strategies and proceduresPublished by Elsevier ,1981
- Attempts to define the antigenic structure of
- Purification, Properties and Amino Acid Sequence of α-Bungarotoxin from the Venom ofBungarus multicinctusHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1972