Production and Properties of Mouse Antihapten Antibodies

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Abstract
Summary: Mice of inbred strains, despite their genetic homogeneity, produced antibodies with several types of molecular heterogeneity. A simple hapten, the p-azobenzoate group, elicited antibodies of the three γG classes. Furthermore, individual mice of the same strain produced anti-p-azobenzoate antibodies of widely different affinity. The antibodies directed against p-azobenzoate groups of a conjugate had a faster electrophoretic mobility than did the antibodies directed against the carrier protein of the same conjugate. This striking characteristic was found in all the strains tested and it was independent of the immunoglobulin class or carrier used. For the production of antibodies against the p-azobenzoate hapten, hemocyanin seems to be a better carrier protein than bovine serum albumin. Peritoneal fluid was used as the source of antibodies and myeloma globulins. Abundant amounts of peritoneal fluid could be almost always induced by the intraperitoneal inoculation of Ehrlich ascites tumor cells, whereas the inoculation of Freund's adjuvant failed to produce ascites in some strains. Antibodies were recovered from ascitic fluid using insoluble specific immunoadsorbents.

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