E1A andrasOncogenes Synergistically Enhance Recombinant Protein Production under Control of the Cytomegalovirus Promoter in BHK-21 Cells
- 1 January 1995
- journal article
- research article
- Published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry
- Vol. 59 (2) , 345-347
- https://doi.org/10.1271/bbb.59.345
Abstract
The amplified ras oncogene greatly enhanced the production of recombinant human interleukin-6 (hIL-6) under control of the cytomegalovirus immediate early promoter (CMV promoter) in BHK-21 cells. When the adenovirus E1A oncogene was further transfected into the above mentioned ras-amplified hIL-6 hyperproducing BHK cells, the transfectants had about 10 times higher productivity than non-transfectants. However, the E1A gene alone did not enhance productivity. These results implicate a ras and E1A synergistic ability that acts to enhance hIL-6 production.Keywords
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