Characterization and Localization of Proopiomelanocortin Messenger RNA in the Adult Rat Testis
- 10 August 1984
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 225 (4662) , 632-634
- https://doi.org/10.1126/science.6740329
Abstract
Northern blot analysis of total RNA and polyadenylated RNA isolated from adult rat testes showed that a proopiomelanocortin (POMC)-like messenger RNA molecule is present in these extracts. The testicular POMC messenger RNA is comparable in length to amygdala and midbrain POMC messenger RNA and appears to be at least 200 nucleotides shorter than POMC messenger RNA found in the hypothalamus and anterior and intermediate lobes of the pituitary gland. Hybridization in situ showed that POMC messenger RNA is located in Leydig cells, which are the only testicular cells that contain immunostainable POMC-derived peptides. These results suggest that local synthesis of POMC occurs in the testis.Keywords
This publication has 22 references indexed in Scilit:
- Identification of proopiomelanocortin neurones in rat hypothalamus by in situ cDNA-mRNA hybridizationNature, 1983
- Variants of alpha-melanocyte stimulating hormone in rat brain and pituitary: Evidence that acetylated α-MSH exists only in the intermediate lobe of pituitaryBiochemical and Biophysical Research Communications, 1982
- β-Endorphin is processed differently in specific regions of rat pituitary and brainNature, 1982
- Acetylated and nonacetylated forms of β-endorphin in rat brain and pituitaryBiochemical and Biophysical Research Communications, 1981
- Corticotropin-like substances in human gastric antrum and pancreasBiochemical and Biophysical Research Communications, 1980
- β-Endorphin in male rat reproductive organsBiochemical and Biophysical Research Communications, 1980
- Distribution of active and inactive forms of endorphins in rat pituitary and brain.Proceedings of the National Academy of Sciences, 1979
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Presence of corticotropin in brain of normal and hypophysectomized rats.Proceedings of the National Academy of Sciences, 1977