New colorimetric method for quantitative determination of protein in urine.
Open Access
- 1 May 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 23 (5) , 811-812
- https://doi.org/10.1093/clinchem/23.5.811
Abstract
Total urinary protein is rapidly precipitated at room temperature by tannic acid. The tannic acid/protein precipitate, dissolved in aqueous triethanolamine/ferric chloride solution, gives a purple-violet color of high absorptivity. Absorbance at 510 nm is linearly related to concentration from 0.05 to 1.50 A for a protein content of 0.05 to 1.50 g/liter, and less than 5 mg/liter can be detected. The CV and analytical recovery ranged from 0.5 to 1.8% and 98 to 103%, respectively. Nonprotein urinary constituents do not interfere.This publication has 1 reference indexed in Scilit:
- Use of a Tannic Acid-Caffeine Concentration Procedure for Detecting Urinary Proteins and HemagglutininsAmerican Journal of Clinical Pathology, 1968