Determination of Chloroquine in Urine by Gas Chromatography

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Abstract
Optimum conditions for the determination of chloroquine in biological materials using gas chromatograph equipped with 10 mCi 63Ni electron capture detector was investigated. A U-shaped glass column (3 mm .times. 2 m) was packed with 3% silicone OV-17 coated Gaschrom Q (mesh 100/120). N2 was used as a carrier gas. The column oven was regulated to 260.degree. C, and the detector oven and the injection port were at 280.degree. C. Chloroquine was extracted with hexane from the standard solution or rabbit urine saturated with potassium carbonate. The calibration curve was drawn with the use of O-ethyl O-(p-nitrophenyl) phenylphosphonothioate as an internal standard. A few nanograms of chloroquine can be determined by this procedure.

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