Incorporation of [14C]histidine into homocarnosine and carnosine of frog brain in vivo and in vitro

Abstract
1. Homocarnosine, carnosine and histidine were determined in brain from several species and the results compared with values in the literature. 2. [14C]Homocarnosine and [14C]carnosine were isolated from frog brain after intracerebral injection of [14C]histidine in vivo. 3. Whole frog brain, incubated in vitro with l-[14C]histidine, formed labelled homocarnosine and carnosine. 4. A frog brain homogenate or supernatant fraction catalysed the incorporation of l-[14C]histidine into homocarnosine and carnosine. 5. The results indicate that brain tissue can synthesize homocarnosine and carnosine.