Genetic polymorphism and close linkage of two α1-protease inhibitors in horse serum
- 24 April 2009
- journal article
- research article
- Published by Wiley in Animal Blood Groups and Biochemical Genetics
- Vol. 10 (4) , 235-251
- https://doi.org/10.1111/j.1365-2052.1979.tb01031.x
Abstract
Two-dimensional electrophoretic analysis of horse serum proteins was done by a 1st-dimension separation in agarose gel (pH 5.4) followed by a 2nd-dimension separation in horizontal polyacrylamide gel (pH 9.0). This method resulted in improved and reproducible separation of many .alpha.-globulins. Two groups of .alpha.1-globulins, designated Pi1 and Pi2, were protease inhibitors. Preliminary studies indicated that the Pi1 and Pi2 proteins differed from each other in MW and in protease inhibiting spectra. Extensive polymorphism was observed for both proteins. Family data supported the hypothesis that Pi1 and Pi2 types were controlled by autosomal codominant alleles. For both Pi1 and Pi2 systems, most of the homozygous types showed 2 fractions each while the heterozygous types had 4 fractions. Six Pi1 and 5 Pi2 alleles were observed in 2 breeds of Swedish horses. Complete genetic linkage was observed for Pi1 and Pi2 loci as no recombinant type was observed in 40 informative matings studied.Keywords
This publication has 22 references indexed in Scilit:
- Close linkage between the albumin and Gc loci in the horseAnimal Blood Groups and Biochemical Genetics, 2009
- A new allele in the prealbumin system of horse serum markersAnimal Blood Groups and Biochemical Genetics, 2009
- Identification and Characterisation of the Major Antiproteases in Equine Serum and an Investigation of their role in the onset of Chronic Obstructive Pulmonary Disease (COPD)Equine Veterinary Journal, 1979
- Interactions in vitro and in vivo between rat serum protease inhibitors and anodal and cathodal rat trypsin and chymotrypsinBiochimica et Biophysica Acta (BBA) - Enzymology, 1979
- Purification and characterization of canine α-1-antiproteinaseBiochemistry, 1978
- Evolutionary conservation of gene linkageNature, 1978
- Identity of Xk and Pa systems in equine serumAnimal Blood Groups and Biochemical Genetics, 1978
- Genetic polymorphism of the vitamin D binding protein and another post‐albumin protein in horse serumAnimal Blood Groups and Biochemical Genetics, 1978
- Horizontal polyacrylamide gradient gel electrophoresis for the simultaneous phenotyping of transferrin, post‐transferrin, albumin and post‐albumin in the blood plasma of cattleAnimal Blood Groups and Biochemical Genetics, 1977
- Characterization of Natural Inhibitors of Trypsin and Chymotrypsin by Electrophoresis in Acrylamide-Agarose GelsNature, 1968