Analysis of developing enamel of the rat

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Abstract
Carbohydrate analyses showed L-fucose, D-xylose, D-mannose, D-arabinose, N-acetyl glucosamine, N-acetyl galactosamine, N-acetyl mannosamine, glucuronic acid and several unknown species to be present in the immature enamel or in one of its fractions. The concentration of carbohydrates was greatest in the water-extractable fraction and next greatest in the water-insoluble fraction. The water-extractable, EDTA-water-soluble, and water-insol-uble fractions each differed in their spectrum of carbohydrates. With DEAE-Sephadex fractionation at pH 5.5, the water-extractable fraction was separated partially into 7 peaks. Four fractions were recovered from the 7 peaks, each of which differed from the others in amino acid and carbohydrate content and in polyacrylamide gel characteristics. Immuno-electrophoretic procedures showed that antibodies will develop against the EDTA-water-soluble fraction.

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