Imaging single-molecule dichroism
- 1 May 1997
- journal article
- Published by Optica Publishing Group in Optics Letters
- Vol. 22 (9) , 651-653
- https://doi.org/10.1364/ol.22.000651
Abstract
Video-enhanced optical fluorescence microscopy has been used to determine the direction of the transition dipole moment of individual molecules. Fast electro-optical switching of the polarization of the excitation allowed us to detect the linear dichroism of single fluorophores in solid phospholipid membranes. Quantitative analysis of the fluorescence signal showed that rotational mobility is absent in solid biomembranes.Keywords
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