Mechanics of Cell Division. I. The Living Spindle

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Abstract
The observation that exposure to X-radiation causes partial dissolution of cell spindles and that chromosomes in the destroyed area become pycnotic led to study of the living spindle. Phase contrast photographs of grasshopper (Melanoplus differentialis) testis, living cells that have been immersed in Belar soln., show spindle fibers and the attachment of some of these fibers from the centrosome to the chromosome. Material injd. with colchicine 24 hours prior to extirpation does not show a spindle or fibers. Cells that have fibers already are not affected in vitro by addition of colchicine to the slide. Reality of the spindle in fixed tissue, as revealed by phase contrast microscopy, is emphasized as a result of these studies. Living cells were observed and photographed throughout a meiotic cycle.

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