Rapid Detection of Polymorphisms of the Nitric Oxide Cascade
- 24 January 2002
- journal article
- research article
- Published by Walter de Gruyter GmbH in cclm
- Vol. 40 (4) , 341-344
- https://doi.org/10.1515/cclm.2002.054
Abstract
NOS3 (endothelial nitric oxide (NO) synthase) and p22phox (subunit of NAD(P)H oxidase) are two genes whose products are involved in formation and degradation of NO, a ubiquitous signaling molecule largely responsible for the maintenance of normal endothelial function. The G894T polymorphism of NOS3 and the C242T polymorphism of p22phox are reportedly associated with numerous cardiovascular diseases. For each polymorphism we developed a rapid and reliable method with the hybridization probes format on the LightCycler and compared it with conventional PCR-restriction fragment length polymorphism (PCR-RFLP) analysis with regard to reliability, duration and cost. The new methods are more reliable, faster and less expensive than PCR-RFLP analysis and therefore represent a significant advantage in the detection of two candidate risk factors for cardiovascular diseases.This publication has 21 references indexed in Scilit:
- A Common Variant of Endothelial Nitric Oxide Synthase (Glu298Asp) Is an Independent Risk Factor for Carotid AtherosclerosisStroke, 2001
- Genotyping Method for Point Mutation Detection in the Endothelial Nitric Oxide Synthase Exon 7 Using Fluorescent Probes. Clinical Validation in Systemic Sclerosis Patientscclm, 2001
- A gp91phox Containing NADPH Oxidase Selectively Expressed in Endothelial Cells Is a Major Source of Oxygen Radical Generation in the Arterial WallCirculation Research, 2000
- Prognostic Impact of Coronary Vasodilator Dysfunction on Adverse Long-Term Outcome of Coronary Heart DiseaseCirculation, 2000
- Relationship of the C242Tp22phox gene polymorphism to angiographic coronary artery disease and endothelial functionAmerican Journal of Medical Genetics, 1999
- Sequence of cDNAs Encoding Components of Vascular Actin Single-stranded DNA-binding Factor 2 Establish Identity to Purα and PurβJournal of Biological Chemistry, 1997
- Simvastatin, an HMG–Coenzyme A Reductase Inhibitor, Improves Endothelial Function Within 1 MonthCirculation, 1997
- Angiotensin-Converting Enzyme Inhibition With Quinapril Improves Endothelial Vasomotor Dysfunction in Patients With Coronary Artery DiseaseCirculation, 1996
- Promotion of Fibroblast Adhesion by Triple-helical Peptide Models of Type I Collagen-derived SequencesJournal of Biological Chemistry, 1996
- Primary structure and unique expression of the 22-kilodalton light chain of human neutrophil cytochrome b.Proceedings of the National Academy of Sciences, 1988