Factors Affecting the Loss of Carbon Monoxide from Stored Blood Samples*

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Abstract
The loss of carbon monoxide (CO) from whole blood or hemolysates has been investigated. Blood samples were exposed to the atmosphere or to a limited volume of air for various storage periods at three temperatures. The initial hemoglobin (Hb) concentration and the percent carboxyhemoglobin (%COHb) saturation were varied in separate experiments. In addition, the effect of repeated exposure of blood to air was evaluated. The %COHb saturation decreased from 80 to 50% following storage of a 1-mL blood sample with 49 mL of air In a sealed container at room temperature for 45 hr. Greater decreases in the %COHb saturation were observed In samples which were exposed to the atmosphere. Lesser, but significant, losses occurred when samples were stored In a refrigerator or freezer. The concentration of Hb In the samples as well as the Initial %COHb saturation were found to influence the decrease In the %COHb saturation.

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