Influence Of Hypoxia And Hypoxia-Reoxygenation On Endothelial P-Selectin Expression
- 1 January 1996
- journal article
- symposium 6
- Published by S. Karger AG in Pathophysiology of Haemostasis and Thrombosis
- Vol. 26 (Suppl. 4) , 177-181
- https://doi.org/10.1159/000217296
Abstract
P-selectin is an endothelial adhesion molecule involved in the initial step of the neutrophil recruitment. We investigated the effect of hypoxia (95% N2, 5% CO2) and of hypoxia-reoxygenation (95% air, 5% CO2) on the expression of P-selectin by human umbilical vein endothelial cells (HUVEC). P-selectin expression was detected by immunolabelling and quantified by flow cytometric analysis. Our data indicate that hypoxia induces an increase in P-selectin expression with a maximum reached after 90 minutes. A hypoxic exposure of 90 minutes results in a highly significant increase compared to normoxia (p < 0.001, n=13). Furthermore, when a reoxygenation period follows 90 minutes of hypoxia, the initially elevated levels of P-selectin are dramatically enhanced with a maximum obtained after 60 minutes of reoxygenation.Keywords
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