The Primary Structure of tRNAIIArg from Brewers' Yeast. 1. Complete Digestions with Pancreatic and T1 Ribonucleases
Open Access
- 1 August 1975
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 56 (2) , 521-526
- https://doi.org/10.1111/j.1432-1033.1975.tb02257.x
Abstract
TRNAIIArg purified from bulk brewers' yeast tRNA by countercurrent distribution followed by two column‐chromatographic steps was completely digested with pancreatic and T1 ribonucleases. Isolations of the products have been carried out either by column chromatography or by high‐voltage electrophoresis. Analyses of the isolated nucleotides and oligonucleotides were in good agreement and indicate that this tRNA is composed of 76 nucleotide residues including 13 minor nucleotides. Overlaps resulting from the end‐products of the two complementary digests led to a sequence of 25 residues. The primary structure of tRNAIIArg has been determined after partial digestion with T1 ribonuclease as described in the following paper.Keywords
This publication has 14 references indexed in Scilit:
- The Primary Structure of tRNAIIArg from Brewers' Yeast. 2. Partial Digestion with Ribonuclease T1 and Derivation of the Complete SequenceEuropean Journal of Biochemistry, 1975
- Structure primaire des tRNAArgIII de levure de bière: I. Hydrolyse totale par les ribonucléases pancréatique et T1Biochimie, 1974
- Purification du tRNAArgII de levure de bièreBiochimie, 1972
- The primary structure of tRNATrp from brewer's yeast: I. Complete digestion with pancreatic ribonuclease and T1 ribonucleaseBiochimie, 1972
- The sequences of nucleotides in tRNAArgIII from brewer's yeastFEBS Letters, 1972
- Nucleotide Sequence of a Lysine Transfer Ribonucleic Acid from Bakers' YeastScience, 1972
- Sequential degradation of nucleic acids. Degradation of Escherichia coli B phenylalanine transfer ribonucleic acidBiochemistry, 1969
- The Separation of Guanosine Oligonucleotides: Use of Urea to Avoid Aggregate FormationJournal of the American Chemical Society, 1963
- Some reactions of adenosine and inosine phosphates in animal tissuesBiochimica et Biophysica Acta, 1953
- Separation of the Phosphoric Esters on the Filter Paper ChromatogramNature, 1949