New Method for Extraction of Milk Phospholipids

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Abstract
-The phospholipid classes vary widely in solubility depending upon their charge, polarity and the solvent employed, thus certain of them would be difficult to recover completely with water-immiscible solvents as used in the Mojon-nier extraction. In the present procedure the whole milk sample was compounded with silicic acid, the mixture placed in a glass chromatogra-phic column, and the phospholipid fraction eluted with 20% (v/v) formic acid in ethyl ether. Lipid phosphorus analysis and thin-layer chromatography were used for the comparison of phospholipid recovery from 10-ml aliquots of milk by each of three methods - the new, the Mojonnier, and the modified Mojonnier (1.5% NaCl added to the milk sample before a Mojonnier extraction). Five trials verified that the modified Mojonnier method and new silicic acid column chromatographic (SACC) method recovered 10.2 and 12.2 mean % respectively more lipid phosphorus than the conventional Mojonnier procedure. When aliquot samples from the phospholipid extracts recovered by the three methods were compared by thin-layer chromatography, minor differences were evident in the lipid patterns, notably in improved recovery of a component corresponding to phosphatidylserine by the SACC method and in superior recovery of lysophosphatides by the Mojonnier and modified Mojonnier methods.