Regulation of Nitrogenase Synthesis in Intact Cells of Rhodospirillum rubrum: Inactivation of Nitrogen Fixation by Ammonia, L-glutamine and L-asparagine

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Abstract
The synthesis of nitrogenase by intact cells of R. rubrum was repressed in N-free media supplemented with L-glutamine or L-asparagine, but was unaffected by the presence of L-glutamate, L-aspartate or L-histidine. Specific activities attained by cultures in supplemented media maintained under Ar-CO2 were 2-3 times higher than those in N-free medium under N2-CO2. A loss in total activity occurred both in cultures growing with N2 after maximum activity was reached, and in cultures maintained under Ar when the gas phase was changed to N2. There was a rapid loss in N-fixing activity when low concentrations of NH4+, L-glutamine or L-asparagine were added to cultures with high activities, but this could be recovered in the absence of demonstrable protein synthesis. During growth, the degree of inactivation brought about by 0.5 mM-inactivator increased to 80-90%, and NH4+ excreted into the medium reached a maximum concentration towards the end of exponential growth.