ACUTE INFLAMMATORY PULMONARY REACTIONS INDUCED BY CHEMOTACTIC FACTORS

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 96  (1) , 71-83
Abstract
Acute inflammatory reactions were produced in hamster lungs by the intrapulmonary instillation of preformed chemotactic mediators (C5fr [complement component 5] and F-Met-Leu-Phe). By the use of 111In and 125I labeling of homologous neutrophils (PMN) and homologous albumin, respectively, highly reproducible and quantitative parameters of the acute inflammatory response could be obtained. The lung responded in a dose-dependent and time-dependent manner to the instillation of preformed chemotactic mediators. The quantitative parameters indicative of PMN [polymorphonuclear leukocytes] influx were more prominent than changes in vascular permeability. The data obtained by the use of radiolabeled PMN were confirmed by observation with light microscopy. Nonchemotactic substances such as human Ig[immunoglobulin]G, serum albumin and C4 failed to induce inflammatory responses in lung. Interestingly, intact C5 instilled into lung was extremely phlogistic, apparently due to hydrolysis once within the lung. These studies provided an approach to reliable quantitative parameters of inflammatory reactions in the lung and emphasize the in vivo biologic effects of chemotactic mediators.