Detection of Streptococcus mutans by PCR amplification of spaP gene
Open Access
- 1 October 1994
- journal article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 41 (4) , 231-235
- https://doi.org/10.1099/00222615-41-4-231
Abstract
Summary Synthetic oligonucleotide primers were used in the polymerase chain reaction (PCR) to amplify a sequence of the spaP gene, which encodes the surface protein antigen I/II of Streptococcus mutans. A DNA fragment of c. 192 bp was amplified from lysed S. mutans cells or isolated DNA. With S. mutans cells, the lower limit of detection was 4-40 cfu. With these primers, 13 reference and 50 clinical strains of S. mutans were identified. Amplification of the 192-bp product was not demonstrated when 41 strains of other streptococcal and non-streptococcal species were tested. The spaP gene PCR has potential for the rapid diagnosis of S. mutans infections.Keywords
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