γ-Aminobutyric Acid Release from Neurons and Glia

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Abstract
Release of 3H-GABA into the medium from bulk-isolated glial cells, neuronal perikarya and synaptosomes [from rabbit brain] was studied, using a continuous perfusion system. The fractions were incubated with 3H-GABA and spontaneous efflux of label in the presence and absence of aminooxyacetic acid (AOAA) was recorded. Unlabeled GABA at micromolar concentrations markedly stimilated 3H-GABA efflux from glia, synaptosomes and neurons. 3H-GABA from all fractions was also markedly sensitive to stimulation by ouabain. Glutamate was effective in stimulating the spontaneous efflux in the presence of AOAA, while superfusion with a Ca+ deprived medium only slightly stimulated the 3H-GABA efflux from neurons and glia.