INFLUENCE OF INJECTION OF CORTISONE-ACETATE ON BLAST TRANSFORMATION AND ANTIBODY-DEPENDENT CELL-MEDIATED CYTOTOXICITY IN RABBIT

  • 1 January 1976
    • journal article
    • research article
    • Vol. C127  (2) , 187-196
Abstract
Inhibition of circulating antibody production by steroids was repeatedly shown in steroid-sensivive animals such as mice or rabbits. After a single injection of 5 mg of cortisone acetate, mouse spleen cells lose their ability to be transformed by B [bone marrow-derived] cell mitogens such as lipopolysaccharides or Nocardia water soluble mitogen. The influence of cortisone on the reactivity of [rabbit] spleen cells to a B cell mitogen (blast transformation and polyclonal stimulation), and their ability to be cytotoxic in the antibody-dependent cell-mediated cytotoxicity assay was studied. The cells which mediate such a cytotoxicity are cortisone-resistant, whereas the B lymphocytes, which can be transformed and polyclonally activated by these mitogens, are sensitive to cortisone treatment.