Preparation of 5- and 6-(aminomethyl)fluorescein
- 1 September 1992
- journal article
- Published by American Chemical Society (ACS) in Bioconjugate Chemistry
- Vol. 3 (5) , 430-431
- https://doi.org/10.1021/bc00017a013
Abstract
5(6)-Carboxyfluorescein is protected as the diacetate then reduced to 5(6)-(hydroxymethyl)fluorescein diacetate. The separated isomers are subjected to a Mitsunobu reaction with dibenzyl imidodicarbonate, yielding diprotected 5- and 6-(aminomethyl)fluorescein diacetate. Methanolysis of the acetates followed by deprotection with HBr/acetic acid gives 5- and 6-(aminomethyl)fluorescein hydrobromide.Keywords
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