Lens Proteins in Intumescent Cataract
- 1 January 1986
- journal article
- research article
- Published by S. Karger AG in Ophthalmic Research
- Vol. 18 (2) , 61-67
- https://doi.org/10.1159/000265416
Abstract
Intumescent cataractous lenses were investigated as to their water content and protein composition. Nuclei were separated from cortices. Water-soluble, water-insoluble, urea-soluble and urea-insoluble portions were quantified and the subunit composition of the water-soluble crystallin fraction was looked at on SDS-gel electrophoresis. Compared to normal lenses it is observed that the water content in intumescent cataract is increased, water-soluble and urea-soluble fractions are diminished, whereas the urea-insoluble portions are augmented. No major changes are noted in the subunit composition of the soluble protein fractions. Particularly, the relative amount of γ-crystallins is diminished in intumescent cataractous lenses.Keywords
This publication has 2 references indexed in Scilit:
- The alpha and gamma crystallin content in aqueous humor of eyes with clear lenses and with cataractsExperimental Eye Research, 1979
- Protein changes in the human lens during development of sentile nuclear cataractBiochimica et Biophysica Acta (BBA) - Protein Structure, 1976