Starch Bioavailability in the Upper Gastrointestinal Tract of Colectomized Rats

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Abstract
Colectomized rats were used to compare the bioavailability of starch from canned peas, kidney beans, lima beans, corn, cooked rice and AIN-76A purified diet. Postoperative weight gains of colectomized and sham-operated rats were not different. Test meals of ∼2 and ∼3.3 g (dry weight) of each food containing chromic oxide (0.6 g/100 g) were administered to 4–6 rats by gastric intubation. Chromium recovery in ileal digesta 10 h after the meal ranged from 94 to 102%. Significant starch (11–15%) from peas, lima beans or kidney beans was recovered in the ileal digesta; 0.2–0.4% of starch from rice, corn or AIN-76A was in the ileal digesta. Pretreatment of kidney or lima beans with a heat-stable endo-α-amylase decreased the starch recovered in the ileal digesta to 2.7–4.3% of that fed; pretreatment with the amylase and pepsin had no further effect. Oligosaccharide extraction, the size of the test meal and the amount of starch did not affect starch bioavailability.