Dissociation of Infective Poliomyelitis Virus from Neutralizing Antibody by Fluorocarbon

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Abstract
Summary: Varying doses of poliomyelitis type I virus were mixed with appropriately diluted homologous rabbit antiserum and incubated at 37°C for 12–30 hr. The resulting noninfectious mixtures were homogenized with fluorocarbon and the amount of dissociated virus was determined by plaque assay. Similarly treated mixtures of virus and normal rabbit serum served as controls for the small losses of infectivity during incubation and homogenization. The use of optimal procedures permitted total recovery of as little as 90 to 270 plaque forming units from mixtures neutralized by an excess of antibody. Homogenization of these mixtures in absence of fluorocarbon did not yield infectious virus, indicating that the fluorocarbon is responsible for dissociation of virus from antibody.