Adaptation of Colorado Tick Fever Virus to Mouse and Developing Chick Embryo

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Abstract
The authors describe adaptation of Colorado tick fever virus to dba and albino Swiss mice. The virus, causing death of the animals 3-5 days after inoculation, has been transferred through 23 consecutive brain-to-brain passages in mice, after which a neutralization test with a sample of convalescent human serum indicated the identity of the mouse brain-adapted virus with the agent responsible for infection in the human. The mouse brain-adapted strain has been used to initiate a series of chick-embryo passages by inoculating into the yolk sac and harvesting the embryos 4 days after inoculation. The virus has been propagated for 12 consecutive egg passages, retaining its immunological identity.

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