Different TBP-associated factors are required for mediating the stimulation of transcriptionin vitroby the acidic transactivator GAL-VP16 and the two nonacidic activation functions of the estrogen receptor

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Abstract
The estrogen receptor (ER) contains two nonacidic transcriptional activation functions, AF-1 and AF-2 (formerly TAF-1 and TAF-2). In this study we show that AF-1 and AF-2 are able to stimulate transcription In vitro in a HeLa cell system when fused to the DNA binding domain of the yeast activator GAL4. We also demonstrate that a factor(s) required for the function of the ER AFs is chromatographlcally separable from a factor(s) necessary for the activity of the acidic activation domain of VP16. Moreover, Immuno-preclpltation experiments using a monoclonal antibody directed against the TATA box binding protein (TBP) Indicate, that these different factors are associated with TBP in distinct TFIID complexes.