Immunofluorescent Localization of Encephalitogenic Protein in Myelin

Open Access
Publisher Website
Google Scholar
Abstract
Summary: A protein preparation extracted from bovine spinal cord by Dr. E. Roboz-Einstein and coworkers induces allergic encephalomyelitis in guinea pigs. Normal human, guinea pig and bovine spinal cord tissue sections were exposed to rabbit antiencephalitogenic protein serum. Similar control sections were treated with normal rabbit serum, absorbed immune rabbit serum or buffered saline. Following the application of fluorescein isothiocyanate-labeled goat antirabbit γ-globulin, only those sections exposed to the specifically immunized rabbit serum showed fluorescent myelin structures. It is concluded that the encephalitogenic protein is localized in myelin, the target tissue of experimental allergic encephalomyelitis, and further that it lacks species specificity.

This publication has 1 reference indexed in Scilit: