Effects of porcine growth hormone and insulin-like growth factor-I (IGF-I) on immunoreactive IGF-binding protein concentration in pigs

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Abstract
A specific radioimmunoasay (RIA) was established for an acid-stable insulin-like growth factor-binding protein (IGF-BP) isolated from porcine serum. The RIA recognizes a GH-dependent 150 kDa protein in porcine serum; therefore we postulate that the acid-stable IGF-BP is a component of the high molecular weight IGF-BP in porcine serum. The IGF-BP concentration was assayed in porcine serum (normal, 2·44 mg/l; hypophysectomized, 0·83 mg/l; serum from a GH-treated pig, 4·72 mg/l), porcine colostrum (2·55 mg/l), milk (0·91 mg/l), and amniotic (1·82–3·14 mg/l), allantoic (2·94–3·58 mg/l) and follicular (2·28 mg/l) fluids. Serum concentrations of IGF-BP were significantly increased (63%) in pigs chronically injected with porcine GH (pGH) (70 μg/kg body weight per day for 17 days). Concentrations of IGF-BP did not change in porcine serum following acute challenges with pGH (10–1000 μg/kg body weight) or IGF-I (4 or 8 mg per pig). This is the first report of a specific RIA for the porcine GH-dependent IGF-BP. Our results indicate that this IGF-BP is found in a wide variety of biological fluids and that its concentration appears to be regulated by pGH but not by IGF-I. Journal of Endocrinology (1989) 120, 153–160

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