EMBOLIC PNEUMONOPATHY INDUCED BY OLEIC-ACID - SYSTEMATIC MORPHOLOGIC STUDY

  • 1 January 1977
    • journal article
    • research article
    • Vol. 87  (1) , 143-158
Abstract
A systematic study of acute and chronic pulmonary lesions resulting from a single i.v. injection of oleic acid and a new fibrosis lung model is proposed: pulmonary interstitial fibrosis is obtained by means of a number of oleic acid i.v. injections. Nineteen adult dogs received 0.045 g/kg or 0.09 g/kg of oleic acid. A systematic morphologic study was carried out after 1, 2, 3, 4, 6, 12, 24 and 48 h and 1, 2 and 4 wk. Eleven other adult dogs received weekly 1 injection of 0.09 g/kg of pure oleic acid over a period of 1-3 mo. Examination of the lung was carried out by means of light and EM and morphometry. An early stage characterized by the formation of thrombosis and cellular necrosis was followed by a repair stage with the proliferation of Type 2 cells and fibrotic foci in the subpleural areas. Lipid staining with Sudan IV allowed the onset and disappearance of lipid-laden macrophages to be ascertained. The late stage showed pulmonary fibrosis. The extent of the lesions is related to the number of oleic acid injections. Since interstitial pulmonary fibrosis invariably appeared, and only 2 dogs out of 11 died, the model is satisfactory for pathologist and physiologist.