Effect of CD8+ T‐cell depletion on bronchial hyper‐responsiveness and inflammation in sensitized and allergen‐exposed Brown–Norway rats
Open Access
- 1 March 1999
- journal article
- Published by Wiley in Immunology
- Vol. 96 (3) , 416-423
- https://doi.org/10.1046/j.1365-2567.1999.00699.x
Abstract
We examined the role of CD8+ T cells in a Brown–Norway rat model of asthma, using a monoclonal antibody to deplete CD8+ T cells. Ovalbumin (OA)‐sensitized animals were given anti‐CD8 antibody (0·5 mg/rat) intravenously 1 week prior to exposure to 1% OA aerosol and were studied 18–24 hr after aerosol exposure. Following administration of anti‐CD8 antibody, CD8+ cells were reduced to +, CD4+ and CD8+ T cells in airways. Anti‐CD8 antibody caused a further increase in allergen‐induced BHR (PP+ and CD4+ T cells in airways were not affected by anti‐CD8 treatment, the level of CD8+ T cells was significantly reduced in sensitized, saline‐exposed animals (PP+ T‐cell depletion, Th1 cytokine expression was significantly reduced (P+ T cells have a protective role in allergen‐induced BHR and eosinophilic inflammation, probably through activation of the Th1 cytokine response.Keywords
This publication has 39 references indexed in Scilit:
- Depletion of CD8+ T cells enhances pulmonary inflammation but not airway responsiveness after antigen challenge in ratsJournal of Allergy and Clinical Immunology, 1996
- Immune regulation: a new role for the CD8+ T cellImmunology Today, 1994
- Depletion of OX-8 lymphocytes from the blood and airways using monoclonal antibodies enhances the late airway response in rats.Journal of Clinical Investigation, 1993
- Synthesis and characterization of rat interleukin-10 (IL-10) cDNA clones from the RNA of cultured OX8− OX22− thoracic duct T cellsBiochemical and Biophysical Research Communications, 1992
- Predominant TH2-like Bronchoalveolar T-Lymphocyte Population in Atopic AsthmaNew England Journal of Medicine, 1992
- Characterization of allergen-induced bronchial hyperresponsiveness and airway inflammation in actively sensitized Brown-Norway ratsJournal of Allergy and Clinical Immunology, 1991
- Differing Lymphokine Profiles of Functional Subsets of Human CD4 and CD8 T Cell ClonesScience, 1991
- Molecular cloning of rat interleukin 4 cDNA and analysis of the cytokine repertoire of subsets of CD4+ T cellsEuropean Journal of Immunology, 1991
- Regulation and deregulation of human IgE synthesisImmunology Today, 1990
- The appearance of hypodense eosinophils during interleukin-2 treatmentJournal of Allergy and Clinical Immunology, 1990