Location of the α-amylase gene in rumenStreptococcus bovis strains distinguished by unstable amylase activity
- 1 April 1995
- journal article
- Published by Springer Nature in Folia Microbiologica
- Vol. 40 (2) , 181-184
- https://doi.org/10.1007/bf02815419
Abstract
Genetic stability of amylase activity after serial subcultivation experiments with amylolytic ruminalStreptococcus bovis strains was investigated. Two strains Amy+ and Amy− were obtained. Loss of amylase activity connected with the loss of plasmid DNA was not found in these strains. The presence of the gene responible for the amylase activity in the chromosome of these strains was revealed by hybridization of the α-amylase gene on pJK108 against chromosomal DNA ofS. bovis andBacillus subtilis after a complete restriction withEcoRI.Keywords
This publication has 14 references indexed in Scilit:
- Catabolite repression of α amylase gene expression in Bacillus subtilis involves a trans‐acting gene product homologous to the Escherichia coli lacl and galR repressorsMolecular Microbiology, 1991
- Site-directed mutagenesis of a catabolite repression operator sequence in Bacillus subtilis.Proceedings of the National Academy of Sciences, 1990
- Cellulose-Dependent and Penicillin-Resistant Plasmids Isolated from Ruminococcus albusAsian-Australasian Journal of Animal Sciences, 1989
- Occurrence of conjugative amylolytic activity in rumen LactobacilliZentralblatt Fur Mikrobiologie, 1989
- Physiological Characteristics and Deoxyribonucleic Acid Relatedness of Human Isolates of Streptococcus bovis and Streptococcus bovis (var.)International Journal of Systematic and Evolutionary Microbiology, 1985
- Compositions and Characteristics of Strains of Streptococcus bovisJournal of Dairy Science, 1984
- A general method for plasmid isolation in lactobacilliCurrent Microbiology, 1984
- A reliable method for the recovery of DNA fragments from agarose and acrylamide gelsAnalytical Biochemistry, 1981
- Isolation of the pMI 10 plasmid from the α-amylase producing strain of Bacillus subtilisMolecular Genetics and Genomics, 1981
- Taxonomy of the Genus Cellulomonas, Based on Phenotypic Characters and Deoxyribonucleic Acid-Deoxyribonucleic Acid Homology, and Proposal of Seven Neotype StrainsInternational Journal of Systematic and Evolutionary Microbiology, 1979