A novel TIMP-insensitive type IV collagen-degrading metalloproteinase from murine metastatic sarcoma cells
- 30 July 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 268 (1) , 39-42
- https://doi.org/10.1016/0014-5793(90)80967-n
Abstract
A novel type IV collagen-degrading metalloproteinase was purified from the conditioned media of a murine metastatic sarcoma cell line. The molecular weight of the purified enzyme was determined to be 100 kDa by SDS-PAGE, while 700 kDa by gel filtration suggesting that the enzyme has a multimer structure. This enzyme degrades type IV collagen, but neither type I collagen nor casein. The failure of trypsin treatment to enhance the enzyme activity suggested that the purified enzyme did not require activation. Although the enzyme seems to be classified as a matrix metalloproteinase, it was inhibited by neither tissue inhibitor of metalloproteinases (TIMP) nor TIMP-2 and thus represents a novel type IV collagen-degrading metalloproteinase.Keywords
This publication has 17 references indexed in Scilit:
- A Sandwich Enzyme Immunoassay for Collagenase Inhibitor Using Monoclonal AntibodiesMatrix, 1989
- Synthesis of Latent Collagenase and Collagenase Inhibitor by Bovine Aortic Medial Explants and Cultured Medial Smooth Muscle CellsConnective Tissue Research, 1989
- High molecular mass type IV collagen‐specific metalloprotease from human carcinoma tissueFEBS Letters, 1988
- Metalloproteinases in endochondral bone formation: Appearance of tissue inhibitor-resistant metalloproteinasesArchives of Biochemistry and Biophysics, 1987
- Human skin fibroblast stromelysin: structure, glycosylation, substrate specificity, and differential expression in normal and tumorigenic cells.Proceedings of the National Academy of Sciences, 1987
- BIOCHEMICAL INTERACTIONS OF TUMOR CELLS WITH THE BASEMENT MEMBRANEAnnual Review of Biochemistry, 1986
- Purification and characterization of a bone metalloproteinase that degrades gelatin and types IV and V collagenBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1985
- Synthesis of a collagenase inhibitor by gingival fibroblasts in cultureBiochimica et Biophysica Acta (BBA) - General Subjects, 1981
- Metastatic potential correlates with enzymatic degradation of basement membrane collagenNature, 1980
- A rapid assay method of collagenase activity using 14C-labeled soluble collagen as substrateBiochimica et Biophysica Acta (BBA) - Enzymology, 1976