31P NMR studies of the solution structure and dynamics of nucleosomes and DNA
- 1 January 1979
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 6 (4) , 1607-1616
- https://doi.org/10.1093/nar/6.4.1607
Abstract
31P NMR studies of 140 base pair DNA fragments in nucleosomes and free in solution show no detectable change in the internucleotide 31P chemical shift or linewidth when DNA is packaged into nucleosomes. Measurements of 31P spin-lattice relaxation times T1 and 31P-[H] nuclear Overhauser enhancements revealed internal motion with a correlation time of about 4 x 10(-10) sec in double helical DNA, both free in solution and bound to nucleosomal core proteins. This result implies greater dynamic mobility in double helical DNA than has previously been supposed.Keywords
This publication has 21 references indexed in Scilit:
- The conformation of DNA and protein within chromatin subunitsFEBS Letters, 1977
- Phosphorus-31 Fourier transform nuclear magnetic resonance study of mononucleotides and dinucleotides. 2. Coupling constantsBiochemistry, 1976
- Temperature dependence of the phosphorus-31 chemical shifts of nucleic acids. A probe of phosphate ester torsional conformationsBiochemistry, 1976
- Conformational study of cyclic and acyclic phosphate esters. CNDO/2 calculations of angle strain and torsional strainJournal of the American Chemical Society, 1976
- 31P chemical shifts in phosphate diester monoanions. Bond angle and torsional angle effectsBiochemical and Biophysical Research Communications, 1975
- Kinky helixNature, 1975
- Proton and phosphorus magnetic relaxation studies on the dynamic structure of single‐stranded polyriboadenylic acidBiopolymers, 1974
- Quantitative determination of conformations of flexible molecules in solution using lanthanide ions as nuclear magnetic resonance probes: Application to adenosine-5′-monophosphateJournal of Molecular Biology, 1974
- Quantitative Determination of Mononucleotide Conformations in Solution using Lanthanide Ion Shift and Broadening NMR ProbesNature, 1971
- The molecular structure of polyadenylic acidJournal of Molecular Biology, 1961