DNA recovery and direct detection of Tn5 sequences from soil
Open Access
- 1 July 1991
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 13 (1) , 21-24
- https://doi.org/10.1111/j.1472-765x.1991.tb00559.x
Abstract
Specific Tn5 sequences inserted in the genome of Enterobacter agglomerans were detected in EcoRI digested DNA directly recovered from soil 70 d after its inoculation with the bacteria, when these were no longer culturable on agar medium. A new method of DNA extraction from soil was used. No amplification of DNA sequences by PCR was needed.Keywords
This publication has 15 references indexed in Scilit:
- Maintenance of plasmids pBR322 and pUC8 in nonculturable Escherichia coli in the marine environmentApplied and Environmental Microbiology, 1990
- Plasmid mobilization from genetically engineered bacteria to members of the indigenous soil microflora in situCurrent Microbiology, 1990
- Plasmid transfer between soil bacteriaPublished by Springer Nature ,1990
- The extraction and purification of microbial DNA from sedimentsJournal of Microbiological Methods, 1987
- A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative BacteriaBio/Technology, 1983
- Location of nitrogen fixation (nif) genes on indigenous plasmids of Enterobacter agglomeransMolecular Genetics and Genomics, 1983