Metabolism of prostaglandin E1 in dog kidneys

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Abstract
1 The biotransformation of prostaglandin E1 (PGE1) was studied in the isolated, perfused dog kidneys. 2 An average 43% of PGE1 was converted into the less polar metabolite I by a single passage through the kidney. As the re-circulation of the perfusate continued, PGE1 was converted not only into metabolite I but also the least polar metabolite II. The velocity of the conversion of PGE1 into metabolite I was significantly greater than that into metabolite II. Usually, six passages elapsed before maximum degradation of PGE1 occurred. 3 Further separation with silicic acid column chromatography and gas-liquid chromatography showed that metabolite II consists of two individual metabolites, metabolite IIa and metabolite IIb. 4 The present study indicates that the kidney biotransforms PGE1 rather rapidly into three metabolites which are less polar than PGE1.