EFFECT OF MAGNESIUM ON ATP-GENERATING AND ATP-UTILIZING REACTIONS IN EHRLICH ASCITES TUMOR-CELLS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 38  (8) , 1101-1114
Abstract
The effect of Mg on the energy metabolism of [mouse] Ehrlich ascites tumor cells was investigated using a method which allows changes of the cellular content of Mg rapidly at constant low Ca concentration. Cells which have lost some of their Mg accumulate lactate slightly faster than nontreated cells. Mg-loading of these cells decreases the glycolytic flux rates to about 20% under aerobic and anaerobic conditions. The corresponding changes of some glycolytic metabolites suggest an inhibition of the HK-PFK[hexokinase-phosphofructokinase]-system. A similar inhibitory effect of Mg on O2-consumption in the absence and presence of glucose to about 20% and 15%, respectively, was observed. Despite the inhibition of the ATP-generating systems, the ATP concentration increases under all conditions investigated, indicating an inhibition of ATP consuming systems. From experiments in the presence of ouabain, which inhibits the aerobic glycolysis to about 40% and 20% in Mg-depleted and Mg-loaded cells, respectively, it is concluded that Mg affects the active monovalent cation transport. Removal of Mg increases the activity of the (Na+-K+)-ATPase and vice versa, presumably via changes of the cell membrane permeability.

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